Details:

10E8

Broad neutralization coverage of HIV by multiple highly potent antibodies
bNAb:10E8 - XML ExportAlternate Names:No alternate names knownRepresentative Structure: 4G6F

3D Structure Viewer: 4G6F
Donor:N152Best neutralization breadth IC50, %:99*
Binding Site:gp41 MPERNeutralization breadth IC80, %:NA
Primary Reference:23151583Best neutralization potency (in μg/ml):0.25*
# of studies in bNAber:3Polyreactivity:Negative in in vitro assay reported
Competition of bNAbs with sCD4 and bNAbs in ELISA:
sCD4b12a2G12F425X5PG9PGT121PGT125PGT130PGT135
NANANANANANANANANANA
Percent viruses neutralized with an IC50 < 50 μg/ml:
StudyABCDFGAEAGAll clades
Chuang2013100*100*98*100*NANA100*100*98*
Huang201297*100*98*100*NANA100*94*97*
Georgiev2013100*100*96*100*NANA100*100*99*
Median IC50 (μg/ml) against viruses neutralized with an IC50 < 50 μg/ml:
StudyABCDFGAEAGAll clades
Chuang20130.82*0.15*0.35*0.38*NANA0.15*0.19*0.35*
Huang20121.06*0.1*0.42*0.31*NANA0.42*0.22*0.35*
Georgiev20130.71*0.16*0.23*0.05*NANA0.05*0.02*0.25*
Sequence Information:
Heavy Chain
PutativeVgeneIGHV 3-15*05
PutativeJgeneIGHJ 1*01
CDR3 length aa22
CDR3sequence aaARTGKYYDFWSGYPPGEEYFQD
Somatic mutations aa27 (28)
Somatic mutations na64 (69)
Mutation frequency na
Mutation frequency aa
InsertionDeletions aa pos
Light Chain
PutativeVgeneIGLV 3-19*01
PutativeJgeneIGLJ 3*02
CDR3 length aa12
CDR3sequence aaCSSRDKSGSRLSVF
Somatic mutations na22
Somatic mutations aa43
Mutation frequency na
Mutation frequency aa
InsertionDeletions aa pos
Ab sequence characteristics were generated using IGMT/HighV-QUEST and IgAT programs.
External Resources:
EMDataBankNA
PDB
4G6F
GenBankLight Chain AA: AFS68396 Light Chain DNA: JX645770
Heavy Chain AA: AFS68395 Heavy Chain DNA: JX645769

Asterisk (*) indicates value are computed by bNAber from IC50 data on the fly

Key/Legend for Neutralization Breadth
Color Description
Neutralization > 90%
90% >= Neutralization > 60%
60% >= Neutralization > 30%
Neutralization < 30%
Neutralization data is not available in bNAber database
Key/Legend for Neutralization Potency
Color Description
Potency < 0.2 μg/ml
0.2 μg/ml <=Potency < 2 μg/ml
2 μg/ml <= Potency < 20 μg/ml
Potency data is not available in bNAber database