Details:

NIH45-46

Broad neutralization coverage of HIV by multiple highly potent antibodies
bNAb:NIH45-46 - XML ExportAlternate Names:No alternate names knownRepresentative Structure: 3U7Y

3D Structure Viewer: 3U7Y 3U7W
Donor:Donor 45Best neutralization breadth IC50, %:86*
Binding Site:CD4bsNeutralization breadth IC80, %:NA
Primary Reference:21764753Best neutralization potency (in μg/ml):0.08*
# of studies in bNAber:5Polyreactivity:Yes
Competition of bNAbs with sCD4 and bNAbs in ELISA:
sCD4b12a2G12F425X5PG9PGT121PGT125PGT130PGT135
NANANANANANANANANANA
Percent viruses neutralized with an IC50 < 50 μg/ml:
StudyABCDFGAEAGAll clades
Scheid2011100*100*85*75*NA86*100*75*86*
Diskin2011100*100*83*67*NA100*100*67*84*
Diskin2013100*100*85*75*NA86*100*75*86*
Georgiev2013100*94*71*NANANA100*100*86*
Huang2014100*92*80*56*NANA94*81*84*
Median IC50 (μg/ml) against viruses neutralized with an IC50 < 50 μg/ml:
StudyABCDFGAEAGAll clades
Scheid20110.03*0.07*0.14*0.03*NA0.06*0.14*0.23*0.08*
Diskin20110.06*0.09*0.17*0.85*NA0.07*0.03*0.6*0.12*
Diskin20130.03*0.09*0.15*0.05*NA0.07*0.09*0.4*0.09*
Georgiev20130.1*0.06*0.09*NANANA0.12*0.02*0.09*
Huang20140.09*0.1*0.13*0.73*NANA0.27*0.22*0.14*
Sequence Information:
Heavy Chain
PutativeVgeneIGHV1-2
PutativeJgeneJ2*01
CDR3 length aa18
CDR3sequence aaTRGKYCTARDYYNWDFEH
Somatic mutations aa40
Somatic mutations na96 (98)
Mutation frequency na
Mutation frequency aa
InsertionDeletions aa pos
Light Chain
PutativeVgeneIgVK3-11
PutativeJgene
CDR3 length aa5
CDR3sequence aaCQQYEFF
Somatic mutations na23
Somatic mutations aa43
Mutation frequency na
Mutation frequency aa
InsertionDeletions aa pos
Ab sequence characteristics were generated using IGMT/HighV-QUEST and IgAT programs.
External Resources:
EMDataBankNA
PDB
3U7Y3U7W
GenBankLight Chain AA: HE584544 Light Chain DNA: HE584544
Heavy Chain AA: HE584543 Heavy Chain DNA: HE584543

Asterisk (*) indicates value are computed by bNAber from IC50 data on the fly

Key/Legend for Neutralization Breadth
Color Description
Neutralization > 90%
90% >= Neutralization > 60%
60% >= Neutralization > 30%
Neutralization < 30%
Neutralization data is not available in bNAber database
Key/Legend for Neutralization Potency
Color Description
Potency < 0.2 μg/ml
0.2 μg/ml <=Potency < 2 μg/ml
2 μg/ml <= Potency < 20 μg/ml
Potency data is not available in bNAber database